The CWHM pharmacology team provides a wide range of preclinical drug discovery and development services and tools designed to enable and inform research projects.  Our research capacity includes in vitro hepatocyte clearance assays (human and rat), determination of plasma protein binding, ADME assays (Absorption, Distribution, Metabolism, and Excretion), biomarker development and bioanalyses using LC/MS/MS.  Working with our strategic partners, we operate and develop relevant in vivo pharmacology disease models and perform pharmacokinetic (PK) and PK/PD (pharmacodynamic) studies.  We operate in state-of-the-art research facilities at Saint Louis University, which includes an AAALAC accredited vivarium.  Our experienced staff consists of former pharmaceutical industry professionals who have advanced projects from lead development through Phase II clinical trials.

We possess the experience and expertise to fulfill a wide range of in vivo and in vitro pharmacology and pharmacokinetic project needs.

In Vivo Pharmacokinetics in Rats

• Blood, urine, and cerebrospinal fluid sampling and tissue collection
• Drug administration routes: intravenous, intraperitoneal, oral, subcutaneous, ICV

Customized Method Development, Validation and Measurement

• Small Molecules
• Peptides
• Metabolite Identification
• Biomarkers

Multiple Sample Preparation Techniques

• Liquid-Liquid Extraction
• Solid Phase Extraction
• Protein Precipitation

Equipment

• Sciex^TM ABI 4000 - LC/MS/MS
• Thermo Finnigan^TM LCQ Deca XP – LC/MS/MS

• Plasma concentration curves
• Plasma clearance and half-Life
• Volume of distribution at steady state
• Bioavailability
• Area under the plasma concentration time curve
• Maximum concentration
• Minimum concentration after repeated dosing
• Time of maximum concentration

• Hepatocytes - rat, dog, human
• Liver Microsomes - rat, dog, human
• Protein binding – mouse, rat, dog, human
• Matrix stability